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Enzyme-linked immunosorbent assays (ELISA) are a very common family of techniques used to measure the presence of proteins in tissues or cell culture samples. Their sensitivity and specificity depends on the types of antibodies used and the concentration of each reagent.
A number of commercial ELISA kits are available that provide the reagents necessary to detect a protein of interest with great sensitivity and specificity. The following are protocols that we have written for some of these kits:
- Cotinine ELISA Protocol (Calbiotech)
- Mouse Active PAI-1 ELISA Protocol (Molecular Innovations)
- Mouse Total Alpha-2-Antiplasmin ELISA Protocol (Molecular Innovations)
- Mouse Total PAI-1 ELISA Protocol (Molecular Innovations)
- Mouse/Rat Estradiol ELISA Protocol (Calbiotech)
- Questions and Answers about ELISA
- Sandwich ELISA Protocol (R&D Systems DuoSet Kit)
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