Youssef Farhat, Protocol Place Author

Youssef Farhat, Protocol Place Author

Youssef Farhat is an M.D./Ph.D. student in the Medical Scientist Training Program at the University of Rochester.  He is currently in the final year of his medical training after having obtained a Ph.D. in Biomedical Engineering in 2015.

As a graduate student, Youssef was a member of Professor Hani Awad’s lab in the Center for Musculoskeletal Research.  His doctoral work aimed to identify molecular targets for the prevention of debilitating tendon adhesions.

In March of 2012, Youssef started the Protocol Place website and its accompanying YouTube Channel in order to convey some of the research tips and lab techniques that he has learned as a graduate research student in an easy and dynamic medium.



July 30, 2015 - Youssef successfully defended his thesis, “Identification of PAI-1 as a Biological Target for the Promotion of Scarless Flexor Tendon Healing,” in fulfillment of the requirements for a Ph.D. in Biomedical Engineering at the University of Rochester.

March 17, 2014 - Youssef presented his research in a Spotlight Session at the Orthopedic Research Society’s Annual Meeting in New Orleans, LA.  The title of his presentation was, “TGF-β1 Decreases Plasmin-Mediated MMP-2 Activity in Flexor Tendon Cells: Implications for Scarless Tendon Repair.”

March 16, 2014 - Youssef and his colleague, Bryan Bobo, were awarded second place in the Orthopaedic Research Society’s Video Outreach Competition for his video, “Working Together for a Better Future?”  Here is the winning video:

February, 2013 - Youssef passed his Ph.D. qualifying examination and has officially started his thesis work!  The title of his thesis proposal was “Assessing the Role of Matrix Turnover Proteins in Models of Flexor Tendon Healing.”  Youssef hopes to understand how scar tissue forms in the hands so that new therapies can be developed to reduce scarring in the hands and other parts of the body.

January, 2013 – Youssef won first place in the Orthopaedic Research Society’s Video Outreach Competition for his video, “Who Cares About Orthopedic Research?”  Here is the winning video:

December, 2013 – Youssef published his first paper in a scientific journal.  In it, he describes a novel method for analyzing gene expression data obtained with a technique called real-time polymerase chain reaction (RT-PCR).  Here is the citation:

Farhat, Y.M., et al. ”Gene expression analysis of the pleiotropic effects of TGF-β1 in an in vitro model of flexor tendon healing.” PLoS One. 2012;7(12):e51411. Epub 2012 Dec 10. PubMed PMID: 23251524; PubMed Central PMCID: PMC3519680. [Click for Article]

October, 2012 – Youssef received his Master’s Degree in Biomedical Engineering.

June, 2012 – Youssef received “Special Recognition for Excellent Work Developing a Laboratory for BME 262” from the department of Biomedical Engineering at the University of Rochester.  Youssef was the first student to teach a lab section for the University’s Cell and Tissue Engineering course.  He designed activities to teach the basics of cell and tissue culture to ~30 BME students.  He also coauthored the course’s first lab manuals and homework assignments along with the course instructor, Professor Hani Awad.

March, 2012 – Youssef initiated the Protocol Place in order to share his experience doing research in the biological sciences with others.


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