Cellular proliferation is an important parameter for many cell and tissue culture experiments. It is often used to characterize the effects of a growth factor, to assess the biocompatibility of a new material or to test the toxicity of a new drug.
The PicoGreen proliferation assay makes use of a dye (PicoGreen) that fluoresces upon interacting with double-stranded DNA (dsDNA). Thus, the fluorescence generated from a sample can be compared with that of a dsDNA standard and used to measure the DNA in that sample. The DNA is assumed to correspond directly with the number of viable cells in that sample.
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Here is the PicoGreen dsDNA Assay Protocol.
