Youssef Farhat, Written 5/2/13, Last updated 8/1/13
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Introduction
The purpose of this protocol is to prepare a 2.0 M NaCl stock solution. NaCl, or table salt, is often added to solutions in order to adjust their tonicity (NaCl composes the majority of the ions in PBS, TBS, and similar buffers). This solution is therefore useful when preparing buffers used in cell and tissue lysis buffers, ELISAs, and Western blots.
In this protocol, we will use solid NaCl (molecular weight: 58.44 g/mol) to make 100 mL of 2.0 M NaCl stock solution. If you would like to use a similar approach to make a different volume of 2 M NaCl, then you might find our Reagents Calculator useful.
Materials
- NaCl (VWR, #BDH0286)
- Deionized water
- Normal lab equipment, such as pipettes, serological pipettes, graduated cylinders, beakers, a stir plate, a stir bar, a scale
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Procedure
- Add 11.69 g of NaCl to an appropriate beaker (100 mL beaker in this case).
- Raise the volume to 100 mL with deionized water.
- Add a stir bar to the beaker and leave it on a stir plate until completely dissolved (~1 min).
- Sterile-filter, if desired, and transfer to an appropriately labeled container.
Citing this Protocol
Farhat, Y. “Reagent Preparation: NaCl (2.0 M).” Protocol Place. May 2013. Accessed on mm/dd/yyyy. <http://protocol-place.com>
