Cell Culture

Cell and tissue culture is essential for most research in the biological sciences.  We have big plans for this topic, including video tutorials, detailed protocols with illustrations, and brief protocols for printing.  All of these are scheduled to be online by Summer 2012.  To receive notifications as this website develops, click “Follow” on the right side of the page.

Here’s a preview of what’s to come:

Lab Equipment (how they work and how to use them)

• Cell culture hood
• Incubator
• Centrifuge
• Microscope
• Pippettors
• Culture dishes, culture flasks

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Sterile Technique

• Tips for Sterile Technique
• Working Under the Culture Hood
• Sterile-Filtering Fluids
• Tips for Clean Pipetting
• Changing Media of Culture Dishes and Flasks

Maintaining Cell Culture

• Cell-Seeded Collagen Gel Protocol
• Coating 6-well or 12-well plates with Collagen
• Making Complete Culture Media
• Thawing Cryopreserved Cells
• Passaging cells
• Counting cells with a hemocytometer