RNA purification is necessary for gene analysis techniques, such as Real-Time Polymerase Chain Reaction (RT-PCR). We have big plans for this topic, including video tutorials, detailed protocols with illustrations, and brief protocols for printing. To receive notifications as this website develops, click “Follow” on the right side of the page.
In any case, here’s what we have so far:
- Measuring RNA with a NanoDrop Spectrophotometer
- RNA Extraction from Collagen Gels Using Qiagen’s RNeasy Kit (a very commonly used kit)
- RNA Extraction from Collagen Gels Using Epoch’s GenCatch Kit (a cheaper alternative to Qiagen’s RNeasy. I found it to yield equally high quality RNA)
- RNA Purification from Monolayer Samples