Basic Lab Techniques

Some basic lab techniques are essential for the performance of any experiments.  These include pipetting, centrifugation, reagent preparation, and many others.  Here are some basic laboratory techniques that we have written about so far:

  1. Concentrating Protein in Media Samples with Centrifugal Devices
  2. Reagent Preparation
    1. Reconstituting and Aliquoting Human GDF-5 (BioVision)
    2. Reconstituting and Aliquoting Mouse GDF-5 (R&D Systems)
    3. Reconstituting and Aliquoting TGF-β1 (R&D Systems)
  3. Stock Solutions
    1. HEPES Stock Solution (0.1 M, pH 7.4)
    2. 20X DPBS Stock Solution
    3. This sildenafil 100mg uk will ensure that any serious complications do not occur when taking the medication to treat erectile dysfunction. If one is feeling libido at an early age is extremely important as they may aggravate with ordine cialis on line the passage of time. However, there are some other points you should notice before thinking cialis tablets uk of the treatment with this sildenafil citrate medicine that-* You should not take the medicine during cardiovascular problem * You should avoid taking this medicine during high blood sugar level, heart problem, arthrosclerosis or vascular problem. Excessive drinking sales uk viagra or smoking can easily have destructive effects on your wellness in addition to sexual performance.

    4. NaCl (2.0 M) Stock Solution
    5. Tris-HCl (1.0 M, pH 7.4) Stock Solution
  4. RNA Purification
    1. Measuring RNA with a NanoDrop Spectrophotometer
    2. RNA Extraction from Collagen Gels Using Qiagen’s RNeasy Kit (a very commonly used kit)
    3. RNA Extraction from Collagen Gels Using Epoch’s GenCatch Kit (a cheaper alternative to Qiagen’s RNeasy.  I found it to yield equally high quality RNA)
    4. RNA Purification from Monolayer Samples

Leave a Reply

Your email address will not be published. Required fields are marked *

You may use these HTML tags and attributes: <a href="" title=""> <abbr title=""> <acronym title=""> <b> <blockquote cite=""> <cite> <code> <del datetime=""> <em> <i> <q cite=""> <strike> <strong>